USDA ARS Northern Plains Agricultural Research Laboratory

 Sunday July 24, 2005

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Molecular Tools for Bio Control, Soil Quality, and Insect Research

Anthony Caesar Barbara Frederick
Plant Pathologist Microbiologist

COLLABORATORS:

TheCan Caesar

Verlan Cochran

Thomas Shanower

Neal Spencer

 

Genetically engineered markings of weed disease fungi aids studies of interactions between insects and plant pathogens.

 

Below DNA-based methods can identify fungal populations in insects and specific gene probes are also used to study the contribution of fungi to soil aggregation.

Photograph of a concoal scanned laser image of a plant pathogenic fungus genetically transformed with a green fluorescent protein

 

Two DNA photographs with the left being a hybridization of a specific probe with DNA extracted from insect associated fungi and the right photograph being a slot blot showing specific hybridization of a DNA probe to soil binding fungus

 

Molecularly-based taxonomy helps identify plant disease causing fungi when traditional methods are not conclusive.

 

Photograph of fungal DNA fingerprinting being using restriction digest patterns to determine relatedness between different plant pathogenic strains

 

 

 

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